Journal : Annales de l'Institut Pasteur ; vol. 1.
Paris : G. Masson, 1887.
Description : 49–62 p., [1 l.] pl. ; ill.: 2 phot. figs., 6 woodcut figs. ; 25 cm.
Photographs : 2 photomicrographs on printed leaf.
Photographer : author, Pierre-Paul-Émile Roux.
Subject : Anaerobic bacteria — Culture ; Roux flasks.
Pasteur and his assistants were constantly refining the laboratory glass they used in their research. A particularly important invention was the specialized flacon that Roux designed in 1880 for making cultures, a glass flask that induced air flow across inoculated tissue and medium. "Roux's flask" made it possible to attenuate the rabies virus and to produce the first vaccine against the pathogen. For this paper, Roux modified his flask to grow anaerobic cultures, and the two photomicrographs shown proved the efficacy of his device, comparing anthrax bacilli extracted from the muscle of a guinea pig, against those that he successfully cultured.